Gene therapy simplified

ژن درمانی Gene Therapy

Simplified global gene expression profiling

• Simple workflow and analysis—the simplicity and speed of PCR enables a rapid method for gene expression profiling with a ≤2-day workflow from sample to analysis. Intuitive bioinformatics through point-andclick run setup and automated secondary data analysis in Torrent SuiteTM Software with an associated plug-in, the AmpliSeqRNA, enables unambiguous gene expression analysis and visualization f...

Simplified method for gene targeting vector construction.

1.Engelthaler, D.M., K.L. Gage, J.A. Montenieri, M. Chu, and L.G. Carter. 1999. PCR detection of Yersinia pestis in fleas: comparison with mouse inoculation. J. Clin. Microbiol. 37:1980-1984. 2.Stevenson, H.L., Y. Bai, M.Y. Kosoy, J.A. Montenieri, J.L. Lowell, M.C. Chu, and K.L. Gage. 2003. Detection of novel Bartonella strains and Yersinia pestis in prairie dogs and their fleas (Siphonaptera: ...

Recent Advances in Hemophilia Gene Therapy

Heterogeneous loss of function mutations at F8 and F9 genes causes X-linked recessive bleeding disorders, hemophilia A (HA) and hemophilia B (HB), respectively. HA is clinically indistinguishable from HB and accounts for more than 80% of hemophilia cases; the former affects 1/5000 and the latter 1/25000 male births worldwide. In Iran, it is estimated that around 4300 HA and 900 HB patients are ...

Beta thalassemia gene therapy using lentiviral vectors

Recent years, allogeneic bone marrow transplantation (BMT) has proved to be the successful cure for patients with thalassemia major, however this is restricted due to limited matched-related donor. Its complications include chronic graft-versus-host disease in 5-8% of patients. So, a molecular approach, such as gene therapy for direct normal beta globin gene transmission, seems quite promising ...